10X TBE buffer

10X TBE buffer ID: OSW73286a25137f44109a893164412eebb4 | UUID: 73286a25-137f-4410-9a89-3164412eebb4
ID	OSW73286a25137f44109a893164412eebb4
UUID	73286a25-137f-4410-9a89-3164412eebb4
Label	10X TBE buffer
Machine compatible name	10xTbeBuffer
Types/Categories	Recipe
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Additional RDF Type(s)	[schema:HowTo schema:HowTo]
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Types/Categories<br>Definition: Entity, Item, Creative Work, How To, Recipe: Recipe Description<br>Definition: Entity: Recipe for 10X TBE (Tris-Borate-EDTA) buffer for nucleic acid electrophoresis. Final composition of 10X: 0.89 M Tris, 0.89 M boric acid, 20 mM EDTA (pH ≈ 8.3). This recipe makes 1 L of 10X stock solution. Store at room temperature; can be autoclaved or filter-sterilized. Label(s)At least one label is required.<br>Definition: Entity: 10X TBE buffer Preparation time<br>Definition: Recipe: 15 minutes Ingredients<br>Definition: Recipe: Tris base — 107.88 g (to give 0.89 M in 1 L 10X) Boric acid — 55.03 g (to give 0.89 M in 1 L 10X) EDTA disodium salt, dihydrate — 7.44 g (to give 20 mM in 1 L 10X) Deionized or distilled water to 1 L Yield<br>Definition: Recipe: 1 Processing time<br>Definition: Recipe: 30 minutes Steps<br>Definition: Recipe: Weigh reagents: Accurately weigh Tris base (≈107.9 g), boric acid (≈55.0 g) and EDTA disodium salt, dihydrate (≈7.44 g) using an analytical balance. Dissolve: Place the solids into a beaker or flask and add about 800 mL of deionized water. Stir until all solids are completely dissolved. Adjust volume: Transfer solution to a 1 L volumetric flask (or graduated cylinder) and add deionized water to bring the final volume to 1 L. Check pH (optional): Measure the pH; 10X TBE is typically around pH 8.3. If necessary, adjust pH carefully with HCl or NaOH. Small adjustments are rarely required if using analytical-grade reagents. Sterilize and store: Sterilize by autoclaving or filter-sterilize using a 0.22 µm filter if required. Label the bottle with concentration and date. Store at room temperature. Prepare working solution: When needed, dilute 10X stock 1:10 with deionized water to obtain 1X TBE (final: 89 mM Tris, 89 mM borate, 2 mM EDTA). Total duration<br>Definition: Recipe: 45 minutes

Description

Recipe for 10X TBE (Tris-Borate-EDTA) buffer for nucleic acid electrophoresis. Final composition of 10X: 0.89 M Tris, 0.89 M boric acid, 20 mM EDTA (pH ≈ 8.3). This recipe makes 1 L of 10X stock solution. Store at room temperature; can be autoclaved or filter-sterilized.

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jsondata

type	"Category:OSW81e9e22e7d934382a6a56df7d3736957"
rdf_type	"schema:HowTo"
uuid	"73286a25-137f-4410-9a89-3164412eebb4"
label	text "10X TBE buffer " lang "en"
description	text "Recipe for 10X TBE (Tris-Borate-EDTA) buffer for nucleic acid electrophoresis. Final composition of 10X: 0.89 M Tris, 0.89 M boric acid, 20 mM EDTA (pH ≈ 8.3). This recipe makes 1 L of 10X stock solution. Store at room temperature; can be autoclaved or filter-sterilized." lang "en"
input	"Tris base — 107.88 g (to give 0.89 M in 1 L 10X)" "Boric acid — 55.03 g (to give 0.89 M in 1 L 10X)" "EDTA disodium salt, dihydrate — 7.44 g (to give 20 mM in 1 L 10X)" "Deionized or distilled water to 1 L"
output_count	1
preparation_duration	"15 minutes"
processing_duration	"30 minutes"
total_duration	"45 minutes"
steps	@type "schema:HowToStep" step_name "Weigh reagents" step_description "Accurately weigh Tris base (≈107.9 g), boric acid (≈55.0 g) and EDTA disodium salt, dihydrate (≈7.44 g) using an analytical balance." @type "schema:HowToStep" step_name "Dissolve" step_description "Place the solids into a beaker or flask and add about 800 mL of deionized water. Stir until all solids are completely dissolved." @type "schema:HowToStep" step_name "Adjust volume" step_description "Transfer solution to a 1 L volumetric flask (or graduated cylinder) and add deionized water to bring the final volume to 1 L." @type "schema:HowToStep" step_name "Check pH (optional)" step_description "Measure the pH; 10X TBE is typically around pH 8.3. If necessary, adjust pH carefully with HCl or NaOH. Small adjustments are rarely required if using analytical-grade reagents." @type "schema:HowToStep" step_name "Sterilize and store" step_description "Sterilize by autoclaving or filter-sterilize using a 0.22 µm filter if required. Label the bottle with concentration and date. Store at room temperature." @type "schema:HowToStep" step_name "Prepare working solution" step_description "When needed, dilute 10X stock 1:10 with deionized water to obtain 1X TBE (final: 89 mM Tris, 89 mM borate, 2 mM EDTA)."
name	"10xTbeBuffer"