10X TBE buffer

10X TBE buffer
ID	OSW73286a25137f44109a893164412eebb4
UUID	73286a25-137f-4410-9a89-3164412eebb4
Label	10X TBE buffer
Machine compatible name	10xTbeBuffer
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Statements (incoming)

Description

Recipe for 10X TBE (Tris-Borate-EDTA) buffer for nucleic acid electrophoresis. Final composition of 10X: 0.89 M Tris, 0.89 M boric acid, 20 mM EDTA (pH ≈ 8.3). This recipe makes 1 L of 10X stock solution. Store at room temperature; can be autoclaved or filter-sterilized.

Item
Type(s)/Category(s)	Recipe

Creative Work

How To
rdf_type<br>Definition: How To	schema:HowTo

Recipe
steps<br>Definition: Recipe	Weigh reagents: Accurately weigh Tris base (≈107.9 g), boric acid (≈55.0 g) and EDTA disodium... Dissolve: Place the solids into a beaker or flask and add about 800 mL of deionized water. Stir u... Adjust volume: Transfer solution to a 1 L volumetric flask (or graduated cylinder) and add deioni... Check pH (optional): Measure the pH; 10X TBE is typically around pH 8.3. If necessary, adjust pH ... Sterilize and store: Sterilize by autoclaving or filter-sterilize using a 0.22 µm filter if requ... Prepare working solution: When needed, dilute 10X stock 1:10 with deionized water to obtain 1X TB...
preparation_duration<br>Definition: Recipe	15 minutes
output_count<br>Definition: Recipe	1
input<br>Definition: Recipe	Tris base — 107.88 g (to give 0.89 M in 1 L 10X) Boric acid — 55.03 g (to give 0.89 M in 1 L 10X) EDTA disodium salt, dihydrate — 7.44 g (to give 20 mM in 1 L 10X) Deionized or distilled water to 1 L
processing_duration<br>Definition: Recipe	30 minutes
total_duration<br>Definition: Recipe	45 minutes

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jsondata

type

"Category:OSW81e9e22e7d934382a6a56df7d3736957"

rdf_type

"schema:HowTo"

uuid

"73286a25-137f-4410-9a89-3164412eebb4"

label

text	"10X TBE buffer "
lang	"en"

description

text	"Recipe for 10X TBE (Tris-Borate-EDTA) buffer for nucleic acid electrophoresis. Final composition of 10X: 0.89 M Tris, 0.89 M boric acid, 20 mM EDTA (pH ≈ 8.3). This recipe makes 1 L of 10X stock solution. Store at room temperature; can be autoclaved or filter-sterilized."
lang	"en"

input

"Tris base — 107.88 g (to give 0.89 M in 1 L 10X)"

"Boric acid — 55.03 g (to give 0.89 M in 1 L 10X)"

"EDTA disodium salt, dihydrate — 7.44 g (to give 20 mM in 1 L 10X)"

"Deionized or distilled water to 1 L"

output_count

1

preparation_duration

"15 minutes"

processing_duration

"30 minutes"

total_duration

"45 minutes"

steps

@type	"schema:HowToStep"
step_name	"Weigh reagents"
step_description	"Accurately weigh Tris base (≈107.9 g), boric acid (≈55.0 g) and EDTA disodium salt, dihydrate (≈7.44 g) using an analytical balance."

@type	"schema:HowToStep"
step_name	"Dissolve"
step_description	"Place the solids into a beaker or flask and add about 800 mL of deionized water. Stir until all solids are completely dissolved."

@type	"schema:HowToStep"
step_name	"Adjust volume"
step_description	"Transfer solution to a 1 L volumetric flask (or graduated cylinder) and add deionized water to bring the final volume to 1 L."

@type	"schema:HowToStep"
step_name	"Check pH (optional)"
step_description	"Measure the pH; 10X TBE is typically around pH 8.3. If necessary, adjust pH carefully with HCl or NaOH. Small adjustments are rarely required if using analytical-grade reagents."

@type	"schema:HowToStep"
step_name	"Sterilize and store"
step_description	"Sterilize by autoclaving or filter-sterilize using a 0.22 µm filter if required. Label the bottle with concentration and date. Store at room temperature."

@type	"schema:HowToStep"
step_name	"Prepare working solution"
step_description	"When needed, dilute 10X stock 1:10 with deionized water to obtain 1X TBE (final: 89 mM Tris, 89 mM borate, 2 mM EDTA)."

name

"10xTbeBuffer"