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Item:OSW73286a25137f44109a893164412eebb4: Difference between revisions
Item:OSW73286a25137f44109a893164412eebb4 (view source)
Revision as of 20:30, 5 January 2026
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{} | { | ||
"type": [ | |||
"Category:OSW81e9e22e7d934382a6a56df7d3736957" | |||
], | |||
"rdf_type": [ | |||
"schema:HowTo" | |||
], | |||
"uuid": "73286a25-137f-4410-9a89-3164412eebb4", | |||
"label": [ | |||
{ | |||
"text": "10X TBE buffer ", | |||
"lang": "en" | |||
} | |||
], | |||
"description": [ | |||
{ | |||
"text": "Recipe for 10X TBE (Tris-Borate-EDTA) buffer for nucleic acid electrophoresis. Final composition of 10X: 0.89 M Tris, 0.89 M boric acid, 20 mM EDTA (pH ≈ 8.3). This recipe makes 1 L of 10X stock solution. Store at room temperature; can be autoclaved or filter-sterilized.", | |||
"lang": "en" | |||
} | |||
], | |||
"input": [ | |||
"Tris base — 107.88 g (to give 0.89 M in 1 L 10X)", | |||
"Boric acid — 55.03 g (to give 0.89 M in 1 L 10X)", | |||
"EDTA disodium salt, dihydrate — 7.44 g (to give 20 mM in 1 L 10X)", | |||
"Deionized or distilled water to 1 L" | |||
], | |||
"output_count": 1, | |||
"preparation_duration": "15 minutes", | |||
"processing_duration": "30 minutes", | |||
"total_duration": "45 minutes", | |||
"steps": [ | |||
{ | |||
"@type": "schema:HowToStep", | |||
"step_name": "Weigh reagents", | |||
"step_description": "Accurately weigh Tris base (≈107.9 g), boric acid (≈55.0 g) and EDTA disodium salt, dihydrate (≈7.44 g) using an analytical balance." | |||
}, | |||
{ | |||
"@type": "schema:HowToStep", | |||
"step_name": "Dissolve", | |||
"step_description": "Place the solids into a beaker or flask and add about 800 mL of deionized water. Stir until all solids are completely dissolved." | |||
}, | |||
{ | |||
"@type": "schema:HowToStep", | |||
"step_name": "Adjust volume", | |||
"step_description": "Transfer solution to a 1 L volumetric flask (or graduated cylinder) and add deionized water to bring the final volume to 1 L." | |||
}, | |||
{ | |||
"@type": "schema:HowToStep", | |||
"step_name": "Check pH (optional)", | |||
"step_description": "Measure the pH; 10X TBE is typically around pH 8.3. If necessary, adjust pH carefully with HCl or NaOH. Small adjustments are rarely required if using analytical-grade reagents." | |||
}, | |||
{ | |||
"@type": "schema:HowToStep", | |||
"step_name": "Sterilize and store", | |||
"step_description": "Sterilize by autoclaving or filter-sterilize using a 0.22 µm filter if required. Label the bottle with concentration and date. Store at room temperature." | |||
}, | |||
{ | |||
"@type": "schema:HowToStep", | |||
"step_name": "Prepare working solution", | |||
"step_description": "When needed, dilute 10X stock 1:10 with deionized water to obtain 1X TBE (final: 89 mM Tris, 89 mM borate, 2 mM EDTA)." | |||
} | |||
], | |||
"name": "10xTbeBuffer" | |||
} | |||